What is NAD+?
Nicotinamide adenine dinucleotide (NAD+) is an essential coenzyme present in all living cells, central to energy metabolism (cellular respiration), DNA repair, and sirtuins-mediated gene expression regulation. NAD+ levels decline significantly with aging. IV and subcutaneous NAD+ infusion clinics have grown substantially for anti-aging, metabolic health, cognitive support, and addiction recovery. Oral precursors (NMN, NR) are widely available as supplements.
Mechanism of Action
Sirtuin (SIRT1–7) co-substrate activation enabling epigenetic regulation and DNA repair; PARP enzyme co-substrate for base excision repair; Mitochondrial Complex I cofactor supporting electron transport chain; CD38 and SARM1 pathway modulation
Target: Sirtuins (SIRT1–7), PARP enzymes, mitochondrial ETC Complex I, CD38
Research Indications
Research explores effects on telomere length and cellular senescence
May support age-related hormonal changes
Early research into cognitive preservation with aging
Research Protocols
These are commonly discussed research protocols — not medical advice. Consult a healthcare provider before use.
| Goal | Dose | Frequency | Route |
|---|---|---|---|
| Starting Dose | Low dose | Per protocol | Varies |
| Standard Dose | Titrated | Per protocol | Varies |
Peptide & Drug Interactions
Always consult your healthcare provider before combining with other compounds. Use our Interaction Checker for reference.
How to Reconstitute & Inject
Use bacteriostatic (BAC) water only. Avoid saline — may cause precipitation. Refrigerate and use within 28 days.
- 1
Remove NAD+ vial from refrigeration and allow to reach room temperature (15–20 minutes)
- 2
Clean vial top with alcohol swab and allow to air dry completely
- 3
Using a sterile syringe, draw the calculated volume of bacteriostatic water (BAC water)
- 4
Inject BAC water slowly down the side of the vial — do not aim directly at the powder
- 5
Gently swirl in circular motions — DO NOT shake vigorously as this degrades the peptide
- 6
Allow 2–3 minutes for full dissolution — solution should be clear and colorless
- 7
Draw calculated dose into an insulin syringe for subcutaneous injection
- 8
Inject into abdomen, thigh, or upper arm. Rotate injection sites to prevent lipodystrophy
- 9
Store reconstituted solution refrigerated (2–8°C) and use within 28 days
What to Expect
Week 1–2
Initial effects and adaptation period. Monitor for any adverse reactions.
Week 2–4
Primary effects begin to manifest. Adjust dosing as recommended by your provider.
Week 4–8
Main therapeutic effects should be established. Assess progress with your provider.
Week 8+
Evaluate results and determine continuation, cycling, or dose adjustments.
Side Effects & Safety
- Injection site reactions (redness, bruising, mild pain)
- Potential for allergic reaction — discontinue if rash or hives occur
- Individual reactions vary — start at the lowest dose and monitor carefully
- Consult your healthcare provider before use and report any unusual symptoms
FDA Status & Regulatory Info
Nicotinamide adenine dinucleotide (NAD+) is an essential coenzyme present in all living cells, central to energy metabolism (cellular respiration), DNA repair, and sirtuins-mediated gene expression regulation. NAD+ levels decline significantly with aging. IV and subcutaneous NAD+ infusion clinics have grown substantially for anti-aging, metabolic health, cognitive support, and addiction recovery. Oral precursors (NMN, NR) are widely available as supplements.
Frequently Asked Questions
Research References
External links for education only. We do not control third-party content.
Community-reported data
The information below reflects self-reported experiences from PeptIQ app users. It is not clinical evidence and should not replace professional medical advice.
Community Intelligence
What 96 users report
Positive 2% · Neutral 98% · Negative 0%
Most reported benefits
Most reported side effects
Dose distribution
Median: 2000+ mcg · Most common: 2000+ mcg
Commonly stacked with
How repeat users are trending
Among repeat reporters, 95% said they felt similar to their last entry, 5% more positive, and 0% more negative.
Overall, repeat reporters leaned more positive than their previous entry.
Median gap between entries: 51 days · Based on 42 repeat reporters
Share Your Experience
Rate NAD+ in the PeptIQ app and help the community make informed decisions.
Research purposes only
This page is for educational reference. It is not medical or legal advice. Consult the FDA and a licensed healthcare professional for current regulations and individualized guidance.