How to Reconstitute Peptides: Step-by-Step Guide with Calculator
Master peptide reconstitution with our comprehensive guide. Learn proper techniques, calculate exact dosages, and avoid common mistakes when mixing peptides with bacteriostatic water.
Understanding Peptide Reconstitution
Reconstitution is the process of mixing a lyophilized (freeze-dried) peptide powder with a sterile liquid to create an injectable solution. This is a critical step that affects the stability, potency, and safety of your peptide.
What You'll Need
- Lyophilized peptide vial
- Bacteriostatic water (BAC water)
- Sterile syringes (typically 1mL insulin syringes)
- Alcohol swabs
- Clean workspace
- Peptide amount: 5mg (5000mcg)
- BAC water added: 2.5mL
- Concentration: 5000mcg ÷ 2.5mL = 2000mcg/mL (or 2mg/mL)
- Wash hands thoroughly
- Clean your workspace with isopropyl alcohol
- Gather all materials
- Allow peptide vial to reach room temperature
- Wipe the rubber stopper of both vials with alcohol swabs
- Draw your calculated amount of BAC water into a syringe
- Insert needle into peptide vial at an angle
- Slowly add water, letting it run down the side of the vial
- Do NOT shake or swirl aggressively
- Let the vial sit for 5-10 minutes
- Gently roll the vial between your palms if needed
- Solution should be clear - if cloudy, do not use
- Vial: 5mg BPC-157
- Water added: 2mL
- Concentration: 2.5mg/mL (2500mcg/mL)
- Desired dose: 250mcg
- Draw: 0.1mL (10 units on insulin syringe)
- Vial: 2mg each peptide
- Water added: 2mL
- Concentration: 1mg/mL (1000mcg/mL)
- Desired dose: 100mcg each
- Draw: 0.1mL (10 units)
- Temperature: 36-46°F (2-8°C) - refrigerator
- Light: Keep away from direct light
- Duration: Most peptides stable 28-42 days
- Handling: Minimize temperature fluctuations
Step-by-Step Reconstitution Process
Step 1: Calculate Your Volume
Before reconstituting, decide how much bacteriostatic water to add. This determines your concentration.
Example Calculation:
Step 2: Prepare Your Workspace
Step 3: Add Bacteriostatic Water
Step 4: Allow to Dissolve
Common Reconstitution Mistakes to Avoid
| Mistake | Why It's Bad | Solution |
| Injecting water directly onto powder | Damages peptide structure | Aim at vial wall |
| Shaking vigorously | Denatures peptides | Roll gently |
| Using non-sterile water | Contamination risk | Always use BAC water |
| Incorrect dosing math | Under/overdosing | Double-check calculations |
Dosage Calculation Examples
Example 1: BPC-157
Example 2: CJC-1295/Ipamorelin
Storage After Reconstitution
Once reconstituted, peptides must be stored properly:
Frequently Asked Questions
Q: Can I use sterile water instead of bacteriostatic water?
A: For single-use, yes. But BAC water contains benzyl alcohol which prevents bacterial growth, making it essential for multi-use vials.
Q: What if my peptide won't dissolve?
A: Give it more time (up to 30 minutes). If still cloudy after gentle rolling, the peptide may be damaged.
Q: How do I know my reconstituted peptide is still good?
A: It should remain clear and colorless. Any cloudiness, particles, or color change means it should be discarded.
Use PeptIQ's Built-in Calculator
The PeptIQ app includes a reconstitution calculator that does all the math for you. Simply enter your peptide amount, desired concentration, and get exact measurements instantly.
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